SEAFOOD utilizing the CHD1Z probe revealed a sturdy sign regarding the distal element of Zq (Fig. 2a), assigning your gene inside bands Zq16-q21, i.e., during the edge regarding the distal heterochromatic musical organization out of Zq (Fig. Three). This is significantly surprising as linkage research experienced placed CHD1Z sixty cM from your IREB1 gene (Fig. Three), which was actually mapped towards the equivalent chromosomal area (29). Duplicated SEAFOOD review alongside probes produced by other areas out of CHD1Z, however, always offered each localization that is sameinformation not really revealed). Perhaps, these types of findings might be revealed by just each area to be truly per spot for the recombination to one that’s unusually contracted at metaphase. The probes also hybridized to CHD1W in some metaphases. This particular sign ended up being regarding the part that is distal to of each hands of this W chromosome; the 2 arms tend to be very nearly indistinguishable alongside ordinary staining methods.
Fig. 2. SEAFOOD localization of this chicken CHD1Z (per) as well as ATP5A1Z (b) genes. (remaining) Hybridization sign. (Appropriate) 4?, 6-Diamidino-2-phenylindole-banding pattern (at grayscale) for the related metaphases. Weiterlesen